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Published October 2009 | public
Journal Article

Bioinformatic analysis of abundant, gender-enriched transcripts of adult Ascaris suum (Nematoda) using a semi-automated workflow platform

Abstract

Expressed sequence tag (EST) data representing transcripts with a high level of differential hybridization in suppressive-subtractive hybridization (SSH)-based microarray analysis between adult female and male Ascaris suum were subjected to detailed bioinformatic analysis. A total of 361 ESTs clustered into 209 sequences, of which 52 and 157 represented transcripts that were enriched in female and male A. suum, respectively. Thirty (57.7%) of the 'female' subset of 52 sequences had orthologues/homologues in other parasitic nematodes and/or Caenorhabditis elegans, 13 (25%) exclusively in other parasitic nematodes and nine (17.3%) had no match in any other organism for which sequence data are currently available; the C. elegans orthologues encoded molecules involved in reproduction as well as embryonic and gamete development, such as vitellogenins and chitin-binding proteins. Of the 'male' subset of 157 sequences, 73 (46.5%) had orthologues/homologues in other parasitic nematodes and/or C. elegans, 57 (37.5%) in other parasitic nematodes only, and 22 (14.5%) had no significant similarity match in any other organism; the C. elegans orthologues encoded predominantly major sperm proteins (MSPs), kinases and phosphatases, actins, myosins and an Ancylostoma secreted protein-like molecule. The findings of the present study should support further genomic investigations of A. suum.

Additional Information

© 2009 Elsevier B.V. Received 27 January 2009; accepted 16 March 2009. Available online 8 April 2009. This workwas supported by the Australian Research Council, the Australian Academy of Science and the Australian–American Fulbright Commission (RBG), the Howard Hughes Medical Institute and WormBase (PWS), the China National Science Funds for Distinguished Young Scientists (grant no. 30225033), the National Natural Science Foundation of China (grant no. 30671578), and the Natural Science Foundation of Guangdong Province (key project, grant no. 36835) (XQZ). CC is the grateful recipient of International Postgraduate Research Scholarship (IPRS) from the Australian Government and a fee-remission scholarship through the University of Melbourne, and the Sir Ian Clunies Ross Prize (2008) and Dr Sue Newton (2009) Award from the Faculty of Veterinary Science, The University of Melbourne. Note: New nucleotide sequence data used in this paper have been deposited in the GenBank database under accession numbers GO254595–GO254953.

Additional details

Created:
August 21, 2023
Modified:
October 19, 2023