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Published December 10, 2009 | public
Journal Article

Quality assurance of PASADENA hyperpolarization for ^(13)C biomolecules

Abstract

Object Define MR quality assurance procedures for maximal PASADENA hyperpolarization of a biological ^(13)C molecular imaging reagent. Materials and methods An automated PASADENA polarizer and a parahydrogen generator were installed. ^(13)C enriched hydroxyethyl acrylate, 1^(-13)C, 2,3,3-d_3 (HEA), was converted to hyperpolarized hydroxyethyl propionate, 1^(-13)C, 2,3,3-d_3 (HEP) and fumaric acid, 1^(-13)C, 2,3-d_2 (FUM) to hyperpolarized succinic acid, 1^(-13)C, 2,3-d_2 (SUC), by reaction with parahydrogen and norbornadiene rhodium catalyst. Incremental optimization of successive steps in PASADENA was implemented. MR spectra and in vivo images of hyperpolarized ^(13)C imaging agents were acquired at 1.5 and 4.7 T. Results Application of quality assurance (QA) criteria resulted in incremental optimization of the individual steps in PASADENA implementation. Optimal hyperpolarization of HEP of P = 20% was achieved by calibration of the NMR unit of the polarizer (B_0 field strength ± 0.002 mT). Mean hyperpolarization of SUC, P = [15.3 ± 1.9]% (N = 16) in D _2O, and P = [12.8 ± 3.1]% (N = 12) in H_2O, was achieved every 5–8 min (range 13–20%). An in vivo ^(13)C succinate image of a rat was produced. Conclusion PASADENA spin hyperpolarization of SUC to 15.3% in average was demonstrated (37,400 fold signal enhancement at 4.7 T). The biological fate of 13C succinate, a normally occurring cellular intermediate, might be monitored with enhanced sensitivity.

Additional Information

© Springer 2009. 28 November 2007 Revised: 24 October 2008 Accepted: 5 November 2008. Published online: 6 December 2008. We thank the following for funding: NIH 1R21 CA118509 (PB), NCI 5R01CA122513 (BDR), NIH 1R01NS048589 (JBH, BDR), Rudi Schulte Research Institute (RSRI) (EYC, JBH), James G. Boswell Fellowship (PB, EYC), American Heart Association (PB), American Brain Tumor Association (PB), Tobacco Related Disease Research Program (PB), NARSAD (KH), Cancer Research and Prevention Foundation (EYC). We thank Dr. Daniel P. Weitekamp and Valerie A. Norton for assistance with the polarizer. We also thank Dr. William Opel for support of PASADENA program at HMRI, Dr. Scott Ross for providing convenient access to 14 T Varian high resolution solution NMR facility of Caltech. JBH thanks Drs. Peter Bachert and Wolfhard Semmler (German Cancer Research Center, DKFZ, Heidelberg, Germany) for PhD supervision. PB and BDR thank Drs. OskarAxelsson, Haukur Johannesson, Magnus Karlsson for advice and training with the parahydrogen polarizer, in Malmo and provided for this work under loan agreement between HMRI and GE Healthcare, established by Dr. Klaes Golman.

Additional details

Created:
August 21, 2023
Modified:
October 19, 2023