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Published May 10, 2009 | Accepted Version + Supplemental Material + Published
Journal Article Open

Birth of ophthalmic trigeminal neurons initiates early in the placodal ectoderm

Abstract

The largest of the cranial ganglia, the trigeminal ganglion, relays cutaneous sensations of the head to the central nervous system. Its sensory neurons have a dual origin from both ectodermal placodes and neural crest. Here, we show that the birth of neurons derived from the chick ophthalmic trigeminal placode begins prior to their ingression (HH11), as early as HH8, and considerably earlier than previously suspected (HH16). Furthermore, cells exiting the cell cycle shortly thereafter express the ophthalmic trigeminal placode marker Pax3 (HH9). At HH11, these postmitotic Pax3+ placode cells begin to express the pan-neuronal marker neurofilament while still in the ectoderm. Analysis of the ectodermal origin and distribution of these early postmitotic neurons reveals that the ophthalmic placode extends further rostrally than anticipated, contributing to neurons that reside in and make a significant contribution to the ophthalmic trigeminal nerve. These data redefine the timing and extent of neuron formation from the ophthalmic trigeminal placode.

Additional Information

© 2009 John Wiley & Sons, Inc. Received 17 October 2008; Revised 27 November 2008; Accepted 15 January 2009. Published Online: 5 March 2009. Grant sponsor: National Institutes of Health; Grant number: R01DE16459. We thank Samuel Ki, Virginia Satterfield, and Dr. Vivian Lee for technical assistance.

Attached Files

Published - McCabe2009p74710.1002cne.22004supp4.tiff

Accepted Version - nihms113945.pdf

Supplemental Material - McCabe2009p74710.1002cne.22004supp1.tiff

Supplemental Material - McCabe2009p74710.1002cne.22004supp2.tiff

Supplemental Material - McCabe2009p74710.1002cne.22004supp3.tiff

Supplemental Material - McCabe2009p74710.1002cne.22004supp5.tiff

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Additional details

Created:
August 21, 2023
Modified:
October 18, 2023